Open AccessConstruction and Characterization of a Human T-Cell Lymphotropic Virus Type 3 Infectious Molecular Clone
Author(s) -
Sébastien Alain Chevalier,
Nga Ling Ko,
Sara Calattini,
Adeline Mallet,
MarieChristine Prévost,
Kylene KehnHall,
John Brady,
Fatah Kashanchi,
Antoine Gessain,
Renaud Mahieux
Publication year - 2008
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00247-08
Subject(s) - biology , clone (java method) , syncytium , virology , transfection , green fluorescent protein , virus , microbiology and biotechnology , hek 293 cells , cell culture , human t lymphotropic virus 1 , provirus , gene , genetics , genome , t cell leukemia
We and others have uncovered the existence of human T-cell lymphotropic virus type 3 (HTLV-3). We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro, and tax/rex as well as minus-strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24(gag) protein is detected in the cell culture supernatant. Transfection of 293T-long terminal repeat (LTR)-green fluorescent protein (GFP) cells with the HTLV-3 clone promotes formation of syncytia, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom