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The characterization and identification of “unknowns” is an often-used  laboratory activity in microbiology courses. As course enrollments  become large, the logistics of providing unknown culture mixes, tubed  and plated culture media, plus incubator and refrigerator space become  quite daunting. A few years ago we discontinued the three-week Unknowns  lab exercise when enrollment reached 240 or so students. In its place we  have developed an hour long, midterm lab practical that incorporates  smear preparation and the Gram stain, biochemical media, rapid tests,  and a streak plate. This easy-to-grade laboratory assessment tool has  taken the place of the more traditional station-to-station lab  practical, and has worked well for the instructors responsible for  administering practicals to 480 students in 13 lab sections over a  two-day period

Using an “Unknown Characterization” Activity as the Midterm Lab Practical in a Large Enrollment, Multi-Lab Section Microbiology Course