Open AccessDevelopment and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella pertussis Infection
Author(s) -
Kazunari Kamachi,
Hiromi Toyoizumi-Ajisaka,
Kohei Toda,
Sann Chan Soeung,
Svay Sarath,
Ya Nareth,
Yoshinobu Horiuchi,
Kazunobu Kojima,
Motohide Takahashi,
Yoshichika Arakawa
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.44.5.1899-1902.2006
Subject(s) - bordetella pertussis , loop mediated isothermal amplification , bordetella , whooping cough , microbiology and biotechnology , virology , medicine , biology , bacteria , vaccination , genetics , dna
We developed a loop-mediated isothermal amplification (LAMP) method to detectBordetella pertussis infection. This LAMP assay detectedB. pertussis with high sensitivity, but not otherBordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis ofB. pertussis infection even in clinical laboratories with no specific equipment.