Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis | Zendy

Corina C. P. M. Bens | Zendy; Andreas Voß | Zendy; Corné H. W. Klaassen | Zendy

Open Access

Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis

Author(s) -

Corina C. P. M. Bens,

Andreas Voß,

Corné H. W. Klaassen

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.44.5.1875-1876.2006

Subject(s) - staphylococcus aureus , biology , pulsed field gel electrophoresis , restriction enzyme , gel electrophoresis , dna methylation , genomic dna , microbiology and biotechnology , methicillin resistant staphylococcus aureus , staphylococcal infections , dna , genetics , bacteria , gene , genotype , gene expression

Genomic DNA from methicillin-resistantStaphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genusStaphylococcus with the recognition sequence CCNGG.

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