Open AccessRapid Detection of Brucella spp. in Blood Cultures by Fluorescence In Situ Hybridization
Author(s) -
Nele Wellinghausen,
Karsten Nöckler,
Anja Sigge,
Melanie Bartel,
Andreas Essig,
Sven Poppert
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.44.5.1828-1830.2006
Subject(s) - brucella , brucellosis , biology , in situ , fluorescence in situ hybridization , in situ hybridization , microbiology and biotechnology , 16s ribosomal rna , bacteria , virology , chemistry , gene , genetics , gene expression , organic chemistry , chromosome
Brucellosis is a severe systemic disease in humans. We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species ofBrucella and that can be applied directly to positive blood cultures.