
Use of Molecular Beacon Probes for Real-Time PCR Detection of Plasmodium falciparum and Other Plasmodium Species in Peripheral Blood Specimens
Author(s) -
Sameer Elsayed,
Katherine Plewes,
Deirdre L. Church,
Barbara L. Chow,
Kunyan Zhang
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.44.2.622-624.2006
Subject(s) - plasmodium falciparum , molecular beacon , plasmodium (life cycle) , biology , virology , molecular probe , apicomplexa , peripheral blood , real time polymerase chain reaction , parasite hosting , malaria , microbiology and biotechnology , immunology , gene , genetics , oligonucleotide , world wide web , computer science
We describe the development and evaluation of a novel pair of real-time, fluorescence-based PCR assays using molecular beacon probes for rapid, sensitive, and specific detection and quantification ofPlasmodium falciparum and otherPlasmodium species organisms.