Open AccessUse of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins
Author(s) -
José Maria Marcelino,
Helena Barroso,
Fátima Gonçalves,
Sofia M. da Silva,
Carlos Novo,
Perpétua Gómes,
Ricardo Jorge Camacho,
Nuno Taveira
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.44.2.607-611.2006
Subject(s) - virology , antigen , glycoprotein , recombinant dna , biology , antibody , human immunodeficiency virus (hiv) , virus , enzyme , envelope (radar) , viral envelope , immunology , microbiology and biotechnology , biochemistry , gene , telecommunications , radar , computer science
A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.