Open AccessDetection and Characterization of Human Group C Rotaviruses in Bangladesh
Author(s) -
Mustafizur Rahman,
Sukalyani Banik,
A. S. G. Faruque,
Koki Taniguchi,
David A. Sack,
Marc Van Ranst,
Tasnim Azim
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.9.4460-4465.2005
Subject(s) - rotavirus , virology , biology , group a , reoviridae , diarrhoeal disease , phylogenetic tree , monophyly , group b , microbiology and biotechnology , diarrhea , rotavirus infections , phylogenetics , virus , gene , medicine , clade , genetics , gastroenterology
Group C rotaviruses were detected by reverse transcription-PCR in 14 (2.3%) of 611 group A rotavirus-negative stool specimens from the patients admitted to the International Centre for Diarrhoeal Disease Research, Bangladesh hospital, Dhaka, Bangladesh, during July to December 2003. The low rate of detection suggested that infection with group C rotaviruses was an uncommon cause of hospitalization due to gastroenteritis. In addition, coinfections with pathogenic enteric bacteria were frequently observed in group C rotavirus-infected patients. Nucleotide sequence comparison of the VP4, VP6, and VP7 genes revealed that the Bangladeshi group C rotaviruses were most similar to Nigerian group C rotavirus strains. Phylogenetic analysis suggested that all human group C rotaviruses, including the strains isolated in our study, clustered in a monophyletic branch, which was distantly related to the branch comprised of animal group C rotaviruses.