Open AccessDynamic Range and Reproducibility of Hepatitis B Virus (HBV) DNA Detection and Quantification by Cobas Taqman HBV, a Real-Time Semiautomated Assay
Author(s) -
Magnus Lindh,
Charles Hannoun
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.8.4251-4254.2005
Subject(s) - taqman , virology , hepatitis b virus , coefficient of variation , genotype , microbiology and biotechnology , reproducibility , real time polymerase chain reaction , dna , biology , virus , medicine , chemistry , chromatography , gene , genetics
The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at ≥1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r 2 = 0.97).