Open AccessAffordable, Abbreviated Roche Monitor Assay for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
Author(s) -
Susan A. Fiscus,
Shu Chen,
Donald R. Hoover,
Melissa Kerkau,
Paul Alabanza,
Som Siharath,
John L. Schmitz,
Newton Kumwenda,
Taha E. Taha
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.8.4200-4202.2005
Subject(s) - serial dilution , virology , human immunodeficiency virus (hiv) , virus , lysis , biology , medicine , microbiology and biotechnology , pathology , alternative medicine
The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.