Open AccessMultiplex PCR for Identification of Seven Streptococcus pneumoniae Serotypes Targeted by a 7-Valent Conjugate Vaccine
Author(s) -
Damien M. O’Halloran,
Mary T. Cafferkey
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.7.3487-3490.2005
Subject(s) - streptococcus pneumoniae , serotype , virology , conjugate , pneumococcal conjugate vaccine , multiplex polymerase chain reaction , microbiology and biotechnology , conjugate vaccine , streptococcaceae , pneumococcal infections , biology , polymerase chain reaction , antibiotics , genetics , gene , mathematical analysis , mathematics
Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.