Open AccessUse of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples
Author(s) -
Shiori Nagashima,
Akihiro Yoshida,
Nao Suzuki,
Toshihiro Ansai,
Tadamichi Takehara
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.6.2948-2951.2005
Subject(s) - suppression subtractive hybridization , biology , biofilm , prevotella , microbiology and biotechnology , real time polymerase chain reaction , polymerase chain reaction , computational biology , bacteria , genetics , gene , complementary dna , cdna library
Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.