Open AccessMultilocus Sequence Typing Directly on DNA from Clinical Samples and a Cultured Isolate To Investigate Linked Fatal Pneumococcal Disease in Residents of a Shelter for Homeless Men
Author(s) -
Andrew Birtles,
Noel D. McCarthy,
Carmen Sheppard,
Harry Rutter,
Malcolm Guiver,
Elizabeth Haworth,
Robert C. George
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.4.2004-2008.2005
Subject(s) - multilocus sequence typing , pneumolysin , streptococcus pneumoniae , meningitis , biology , typing , microbiology and biotechnology , blood culture , polymerase chain reaction , dna sequencing , virology , genetics , dna , medicine , gene , genotype , pediatrics , antibiotics
Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.