Open AccessComparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, Including Confirmation with N. gonorrhoeae- Specific 16S rRNA PCR, with Traditional Culture
Author(s) -
D. Luijt,
Petra A. J. Bos,
A. A. Van Zwet,
Pieter Vader,
J Schirm
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.3.1445-1447.2005
Subject(s) - neisseria gonorrhoeae , neisseriaceae , 16s ribosomal rna , microbiology and biotechnology , biology , polymerase chain reaction , predictive value , virology , bacteria , medicine , antibiotics , gene , genetics
A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.