Open AccessCharacterization ofMycobacterium capraeIsolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping
Author(s) -
Wolfgang M. Prodinger,
Anita Brandstätter,
Ludmila Naumann,
M. L. Pacciarini,
Tanja Kubica,
María Laura Boschiroli,
Alicia Aranaz,
G. Nagy,
Željko Cvetnić,
M. Ocepek,
Artem Skrypnyk,
W Erler,
Stefan Niemann,
I. Pavlík,
Irmgard Moser
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.10.4984-4992.2005
Subject(s) - genotyping , microbiology and biotechnology , biology , virology , genotype , genetics , gene
Mycobacterium caprae , a recently defined member of theMycobacterium tuberculosis complex, causes tuberculosis among animals and, to a limited extent, in humans in several European countries. To characterizeM. caprae in comparison with otherMycobacterium tuberculosis complex members and to evaluate genotyping methods for this species, we analyzed 232M. caprae isolates by mycobacterial interspersed repetitive unit (MIRU) genotyping and by spoligotyping. The isolates originated from 128 distinct epidemiological settings in 10 countries, spanning a period of 25 years. We found 78 different MIRU patterns (53 unique types and 25 clusters with group sizes from 2 to 9) but only 17 spoligotypes, giving Hunter-Gaston discriminatory indices of 0.941 (MIRU typing) and 0.665 (spoligotyping). For a subset of 103M. caprae isolates derived from outbreaks or endemic foci, MIRU genotyping and IS6110 restriction fragment length polymorphism were compared and shown to provide similar results. MIRU loci 4, 26, and 31 were most discriminant inM. caprae , followed by loci 10 and 16, a combination which is different than those reported to discriminateM. bovis best.M. caprae MIRU patterns together with published data were used for phylogenetic inference analysis employing the neighbor-joining method.M. caprae isolates were grouped together, closely related to the branches of classicalM. bovis ,M. pinnipedii ,M. microti , and ancestralM. tuberculosis , but apart from modernM. tuberculosis. The analysis did not reflect geographic patterns indicative of origin or spread ofM. caprae. Altogether, our data confirmM. caprae as a distinct phylogenetic lineage within theMycobacterium tuberculosis complex.