Open AccessComparison of the Real-Time PCR Method and the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test for Detection of Mycobacterium tuberculosis in Pulmonary and Nonpulmonary Specimens
Author(s) -
Nadine Lemaître,
Sylvie Armand,
Anne Vachée,
Odile Capilliez,
Christine Dumoulin,
René Courcol
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.9.4307-4309.2004
Subject(s) - mycobacterium tuberculosis , tuberculosis , mycobacterium , microbiology and biotechnology , real time polymerase chain reaction , polymerase chain reaction , mycobacterium tuberculosis complex , biology , medicine , pathology , gene , genetics
Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.