Open AccessDetection of Human Papillomavirus DNA in Cervical Samples: Analysis of the New PGMY-PCR Compared To the Hybrid Capture II and MY-PCR Assays and a Two-Step Nested PCR Assay
Author(s) -
Lucia Giovannelli,
Anna Lama,
Giuseppina Capra,
Viviana Giordano,
Pietro Aricò,
Pietro Ammatuna
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.8.3861-3864.2004
Subject(s) - nested polymerase chain reaction , polymerase chain reaction , biology , hybrid capture , virology , human papillomavirus , microbiology and biotechnology , papillomaviridae , dna , real time polymerase chain reaction , cervical cancer , gene , medicine , cancer , genetics , cervical intraepithelial neoplasia
The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.