Open AccessComparison of a New Lateral-Flow Chromatographic Membrane Immunoassay to Viral Culture for Rapid Detection and Differentiation of Influenza A and B Viruses in Respiratory Specimens
Author(s) -
Andreea C. Cazacu,
Gail J. Demmler,
M A Neuman,
Betty A. Forbes,
Sooyoung E. Chung,
Jewel M. Greer,
A. Álvarez,
Robert B. Williams,
Nadine Y. Bartholoma
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.8.3661-3664.2004
Subject(s) - immunoassay , virus , virology , orthomyxoviridae , predictive value , influenza a virus , respiratory system , viral disease , viral culture , biology , medicine , immunology , antibody
The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.