Open AccessMolecular Fingerprinting ofMycobacterium bovissubsp.capraeIsolates from Central Europe
Author(s) -
W Erler,
G. Martin,
Konrad Sachse,
Ludmila Naumann,
Dagmar I. Kahlau,
J Beer,
Milan Bartoš,
G. Nagy,
Željko Cvetnić,
Manca Žolnir-Dovč,
I. Pavlík
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.5.2234-2238.2004
Subject(s) - restriction fragment length polymorphism , biology , mycobacterium bovis , outbreak , molecular epidemiology , genotype , serotype , dna profiling , microbiology and biotechnology , virology , veterinary medicine , genetics , mycobacterium tuberculosis , tuberculosis , medicine , gene , dna , pathology
To study the dissemination of Mycobacterium bovis subsp. caprae, 79 European isolates from cattle, humans, and other hosts were examined by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) analysis. Among a total of 11 different spoligotypes identified, type C1 proved to be predominant (n = 62). Five of the spoligotypes are described for the first time. A total of 43 different RFLP types were identified, thus allowing further differentiation for epidemiological tracking. Isolates from a series of outbreaks in one village proved to be of the same spoligotype and of identical or closely related RFLP types.