Open AccessPrimer Pair p289-p290, Designed To Detect Both Noroviruses and Sapoviruses by Reverse Transcription-PCR, Also Detects Rotaviruses by Cross-Reactivity
Author(s) -
Juan E. Ludert,
Ana C. Alcalá,
Ferdinando Liprandi
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.2.835-836.2004
Subject(s) - rotavirus , amplicon , virology , biology , primer (cosmetics) , reverse transcriptase , sapovirus , genome , norovirus , gene , polymerase chain reaction , genetics , virus , microbiology and biotechnology , chemistry , organic chemistry
A primer pair (p289-p290) designed to detect both noroviruses and sapoviruses by reverse transcription-PCR (Jiang et al., J. Virol. Methods 83:145, 1999) cross-reacts with rotaviruses. The rotavirus amplicon corresponds to genome segment 1. Furthermore, primer pair p289-p290 detected rotaviruses as efficiently as rotavirus-specific primers directed to rotavirus gene 4.