Open AccessIdentification and Discrimination of Burkholderia pseudomallei , B. mallei , and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes
Author(s) -
François M. Thibault,
Eric Valade,
Dominique Vidal
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.12.5871-5874.2004
Subject(s) - burkholderia pseudomallei , melioidosis , biology , microbiology and biotechnology , burkholderia , gene , bacteria , identification (biology) , polymerase chain reaction , secretion , genetics , biochemistry , botany
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.