Open AccessEvaluation of a Candidate International Standard Preparation for Human Anti- Toxoplasma Immunoglobulin G
Author(s) -
Peter Rigsby,
Sjoerd Rijpkema,
Edward Guy,
Janet Francis,
Rose Gaines Das
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.11.5133-5138.2004
Subject(s) - antibody , immunoglobulin g , virology , biology , microbiology and biotechnology , medicine , immunology
A freeze-dried human serum preparation containing immunoglobulin G (IgG) to Toxoplasma gondii was assessed for its suitability as an international reference reagent in an international collaborative study by 24 laboratories from 17 countries. This candidate standard was compared with the third international standard (IS) for human anti-Toxoplasma serum, TOXM, with the previous second IS, TOXS, and with a range of other serum samples. Samples were tested with the Sabin-Feldman dye test and a range of agglutination assays and enzyme immunoassays. This study emphasizes the need for appropriate standards if intermethod agreement of estimates is to be achieved. On the basis of the results of this study, the preparation was established by the World Health Organization as the first IS for human anti-Toxoplasma IgG, with an assigned potency of 20 IU per ampoule of total anti-Toxoplasma antibodies.