Open AccessComparison of an Immunochromatography Test with Multiplex Reverse Transcription-PCR for Rapid Diagnosis of Respiratory Syncytial Virus Infections
Author(s) -
Yuki Kuroiwa,
Koutatsu Nagai,
Lisa Okita,
Susumu Ukae,
Toshihiko Mori,
Tomoyuki Hotsubo,
Hiroyuki Tsutsumi
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.10.4812-4814.2004
Subject(s) - gold standard (test) , virology , multiplex , multiplex polymerase chain reaction , reverse transcription polymerase chain reaction , virus , medicine , respiratory system , pcr test , reverse transcriptase , polymerase chain reaction , biology , gene , messenger rna , bioinformatics , biochemistry
A new commercial rapid 10-min one-step immunochromatography (IC) test, SAS RSV test, was compared to another IC test, Directigen EZ RSV, employing RT-PCR as the "gold standard" for detecting respiratory syncytial virus. Of 102 clinical samples, 79 were positive by RT-PCR, 66 (82.5%) were positive with the SAS RSV test, and 55 (69.6%) were positive with Directigen EZ RSV. The specificity of the new test was 91.3% (21 of 23), similar to that of Directigen EZ RSV (100% [23 of 23]). This test performs well enough to be used for patient care.