Open AccessSpecific Oligonucleotide Primers for Identification ofCladophialophora carrionii, a Causative Agent of Chromoblastomycosis
Author(s) -
Paride Abliz,
Kazutaka Fukushima,
Kayoko Takizawa,
Kazuko Nishimura
Publication year - 2004
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.42.1.404-407.2004
Subject(s) - chromoblastomycosis , amplicon , cryptococcus neoformans , primer (cosmetics) , biology , microbiology and biotechnology , identification (biology) , polymerase chain reaction , genetics , gene , botany , chemistry , organic chemistry
Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii.