Open AccessPopulation Dynamics of Persistent Staphylococcus aureus Isolated from the Airways of Cystic Fibrosis Patients during a 6-Year Prospective Study
Author(s) -
Barbara C. Kahl,
Angelika Duebbers,
Gabriele Lubritz,
Johannes Haeberle,
Hans Georg Koch,
Barbara Ritzerfeld,
Marion Reilly,
Erik Harms,
Richard A. Proctor,
Mathias Herrmann,
Georg Peters
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.9.4424-4427.2003
Subject(s) - cystic fibrosis , staphylococcus aureus , micrococcaceae , typing , microbiology and biotechnology , biology , population , staphylococcal infections , colonization , immunology , medicine , bacteria , genetics , environmental health
Molecular typing of normal (n = 456) and small-colony-variant (SCV; n = 239) Staphylococcus aureus isolates cultured from the airways of 52 of 72 cystic fibrosis (CF) patients (72.2%) during a 6-year prospective study revealed a median long-term persistence of 37 months (range, 6 to 70). SCV persisted longer in the airways than the normal S. aureus (statistically not significant). Pulsed-field gel electrophoresis identified six prevalent clonal lineages, which were cultured from more than one patient (3 to 12 patients), and 39 individual clones, which were isolated only from single patients. The SCV phenotype was not restricted to a distinct clonal lineage but occurred in many different clones. Most patients (33 of 52, 63.46%) harbored single clones. This study provides a basis for improved understanding of S. aureus colonization and infection dynamics in CF patients.