Open AccessMolecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France
Author(s) -
Béatrice Nicollier-Jamot,
Valérie Pico,
Pierre Pothier,
Évelyne Kohli
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.8.3901-3904.2003
Subject(s) - antigenicity , norovirus , virology , seroprevalence , recombinant dna , virus , biology , cloning (programming) , epitope , antibody , gene , serology , genetics , computer science , programming language
Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.