Open AccessGenotyping by Amplified Fragment Length Polymorphism Analysis Reveals Persistence and Recurrence of Infection with Streptococcus anginosus Group Organisms
Author(s) -
Jan Jacobs,
Jeroen H. T. Tjhie,
Monique G. J. Smeets,
Corrie S. Schot,
Leo M. Schouls
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.7.2862-2866.2003
Subject(s) - streptococcus anginosus , amplified fragment length polymorphism , biology , microbiology and biotechnology , genotyping , streptococcus , streptococcaceae , streptococcus intermedius , genotype , genetics , bacteria , gene , genetic diversity , medicine , population , antibiotics , environmental health
Streptococcus anginosus ,Streptococcus constellatus , andStreptococcus intermedius , commonly referred to as theStreptococcus anginosus group (SAG), are commensal organisms known for their propensity to cause purulent infections which are difficult to eradicate. In this study, we determined the genetic similarities between SAG isolates consecutively recovered from single patients to assess the duration of infection or colonization. A total of 97 SAG isolates recovered from 30 patients were included; 65 (67.0%) of the isolates were abscess related. The isolates were identified by the 16S rRNA reverse line blot hybridization assay asS. anginosus (n = 34),S. constellatus (n = 55), andS. intermedius (n = 8). Amplified fragment length polymorphism (AFLP) analysis of the SAG isolates produced discriminatory and reproducible patterns. Consecutive SAG isolates with identical AFLP types were found in 27 of 30 (90.0%) patients, and consecutive isolates with only a single AFLP type were demonstrated in 21 (70.0%) patients. The median delay between the times of recovery of the first and last isolates of identical AFLP types from each patient was 36 days, and this delay extended for more than 1 year in patients with both colonizing and abscess-related SAG isolates. In six bacteremic patients, paired blood and nonblood SAG isolates showed identical AFLP types.