Open AccessEvaluation of a Fluorescence-Based PCR Method for Identification of Serogroup A Meningococci
Author(s) -
Mathew Diggle,
Karen Smith,
E. K. Girvan,
Stuart C. Clarke
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.4.1766-1768.2003
Subject(s) - neisseria meningitidis , meningococcal disease , virology , polymerase chain reaction , neisseriaceae , biology , microbiology and biotechnology , meningococcal infections , identification (biology) , gene , bacteria , genetics , antibiotics , botany
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.