Open AccessDetection of Hepatitis B Virus DNA in Sera from Patients with Chronic Hepatitis B Virus Infection by DNA Microarray Method
Author(s) -
Kazunori Kawaguchi,
Shuichi Kaneko,
Masao Honda,
Hiroshi Kawai,
Yukihiro Shirota,
Kenichi Kobayashi
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.4.1701-1704.2003
Subject(s) - hepatitis b virus , dna , complementary dna , microbiology and biotechnology , virology , dna microarray , biology , microarray , hepadnaviridae , virus , polymerase chain reaction , real time polymerase chain reaction , hepatitis b virus dna polymerase , gene , gene expression , genetics
We have developed a sensitive and quantitative assay using a DNA microarray for the detection of hepatitis B virus (HBV) DNA in serum. Fluorescently labeled target cDNA prepared from cloned HBV DNA or serum HBV DNA was hybridized to capture DNA on a slide. A linear relationship was obtained between the intensities of the array spot and the amount of the cloned or serum HBV DNA, indicating the quantitative accuracy of this assay system. In addition, there was a significant correlation between the number of molecules of serum HBV DNA determined by the DNA microarray and that determined by a branched-DNA assay (n = 21, r = 0.89). Given these results, we conclude that the DNA microarray assay system may be useful as a diagnostic technique in the clinical laboratory.