Open AccessDirect Identification of Staphylococcus aureus from Positive Blood Culture Bottles
Author(s) -
Kenneth Oliveira,
Stephen M. Brecher,
Annette Durbin,
Daniel S. Shapiro,
Donald R. Schwartz,
Paola C. De Girolami,
Joanna Dakos,
Gary W. Procop,
Deborah A. Wilson,
Chad S. Hanna,
Gerhard Haase,
Heidrun Peltroche-Llacsahuanga,
Kimberle Chapin,
Michael C. Musgnug,
Michael H. Levi,
Cynthia Shoemaker,
Henrik Stender
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.2.889-891.2003
Subject(s) - staphylococcus aureus , blood culture , microbiology and biotechnology , fish <actinopterygii> , medicine , staphylococcus , bacteria , biology , antibiotics , genetics , fishery
Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targetingStaphylococcus aureus 16S rRNA is a novel method for direct identification ofS. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison ofS. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity ofS. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded thatS. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems.