Open AccessIdentification of Dermatophyte Species by 28S Ribosomal DNA Sequencing with a Commercial Kit
Author(s) -
Béatrice Alice Bescher Ninet,
Isabelle Jan,
Olympia Bontems,
Barbara Léchenne,
Olivier Jousson,
Renato G. Panizzon,
Daniel Pablo Lew,
Michel Monod
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.2.826-830.2003
Subject(s) - dermatophyte , biology , ribosomal dna , ribosomal rna , trichophyton , dna sequencing , internal transcribed spacer , dna , microbiology and biotechnology , genetics , phylogenetics , gene , antifungal
We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.