Open AccessFalse Identification of Coccidioides immitis : Do Molecular Methods Always Get It Right?
Author(s) -
B. Cherie Millar,
Jianguo Xu,
Michael J. Walker,
James P. Evans,
John E. Moore
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.12.5778-5780.2003
Subject(s) - coccidioides immitis , internal transcribed spacer , coccidioides , biology , sequence analysis , ribosomal rna , gene , identification (biology) , sequence (biology) , genetics , chrysosporium , microbiology and biotechnology , botany , lignin
rRNA sequence analysis of a partial region of the 18S and 5.8S-internal transcribed spacer 2 (ITS2) region of Chrysosporium keratinophilum highlights its potential molecular misidentification as Coccidioides immitis. Molecular identification of medically important fungi should not be based solely on sequence analysis of the 18S rRNA gene but should be confirmed by sequence analysis of an additional rRNA gene locus, such as the ITS region(s).