Open AccessEvaluation of a PCR-Based Universal Heteroduplex Generator Assay as a Tool for Rapid Detection of Multidrug-ResistantMycobacterium tuberculosisin Peru
Author(s) -
Holger Mayta,
Robert H. Gilman,
Fanny Arenas,
Teresa Valencia,
Luz Caviedes,
Sonia Montenegro,
Eduardo Ticona,
Jaime Ortiz,
Rosa Chumpitaz,
Carlton A. Evans,
Diana L. Williams
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.12.5774-5777.2003
Subject(s) - rpob , heteroduplex , mycobacterium tuberculosis , tuberculosis , biology , virology , multiple drug resistance , drug resistance , polymerase chain reaction , microbiology and biotechnology , gene , genetics , medicine , pathology
Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.