Open AccessComparison of PCR-Enzyme-Linked Immunosorbent Assay and Real-Time PCR Assay for Diagnosis of an Unusual Case of Cerebral Toxoplasmosis in a Stem Cell Transplant Recipient
Author(s) -
Jean Menotti,
Gustavo Vilela,
S. Romand,
Y. J. F. Garin,
Lionel Adès,
Éliane Gluckman,
Francis Derouin,
Patricia Ribaud
Publication year - 2003
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.41.11.5313-5316.2003
Subject(s) - buffy coat , toxoplasmosis , biology , polymerase chain reaction , real time polymerase chain reaction , virology , microbiology and biotechnology , toxoplasma gondii , stem cell , immunology , antibody , gene , genetics
A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.