Open AccessQuantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology
Author(s) -
Yuqi Zhao,
Min Yu,
Johann W. Miller,
Mingzhong Chen,
E. Bremer,
William Kabat,
Ram Yogev
Publication year - 2002
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.40.2.675-678.2002
Subject(s) - taqman , virology , dna , biology , virus , human immunodeficiency virus (hiv) , polymerase chain reaction , rna , provirus , gene , genetics , genome
A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.