Open AccessDiscrimination of Single-Copy IS6110DNA Fingerprints ofMycobacterium tuberculosisIsolates by High-Resolution Minisatellite-Based Typing
Author(s) -
Ann S. G. Lee,
Lynn L. H. Tang,
Irene H. K. Lim,
Richard Bellamy,
Serre-Yu Wong
Publication year - 2002
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.40.2.657-659.2002
Subject(s) - minisatellite , typing , mycobacterium tuberculosis , biology , genetics , restriction fragment length polymorphism , dna profiling , virology , polymerase chain reaction , dna , microbiology and biotechnology , gene , tuberculosis , allele , medicine , microsatellite , pathology
Seven isoniazid-resistant isolates with mutations in the NADH dehydrogenase (ndh) gene were molecularly typed by IS6110-based restriction fragment length polymorphism analysis. All seven isolates with the R268H mutation had identical 1.4-kb IS6110 fingerprints. High-resolution minisatellite-based typing discriminated five of these isolates; two isolates were identical.