Open AccessAssessment of a Combined Testing Strategy for Detection of Antibodies to Human Herpesvirus 8 (HHV-8) in Persons with Kaposi's Sarcoma, Persons with Asymptomatic HHV-8 Infection, and Persons at Low Risk for HHV-8 Infection
Author(s) -
Corey Casper,
Elizabeth M Krantz,
H Taylor,
Julie Dalessio,
David Carrell,
Anna Wald,
Lawrence Corey,
Rhoda Ashley
Publication year - 2002
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.40.10.3822-3825.2002
Subject(s) - human herpesvirus , immunoassay , saliva , asymptomatic , virology , immunofluorescence , antibody , herpesviridae , gammaherpesvirinae , human herpesvirus 6 , biology , immunology , medicine , viral disease , virus , pathology
The performance of a human herpesvirus 8 (HHV-8) enzyme immunoassay (EIA) and selective subsequent use of an HHV-8 immunofluorescence assay (IFA) was tested in persons unlikely to be infected with HHV-8 and those who had HHV-8 detected in their saliva. The IFA was performed on samples within a range of EIA optical densities (0.15 to 0.35) where there was considerable overlap between HHV-8-infected and uninfected individuals. The sensitivity of the testing strategy was 88%, with a specificity of 97%.