Open AccessDirect Detection and Differentiation of Legionella spp. and Legionella pneumophila in Clinical Specimens by Dual-Color Real-Time PCR and Melting Curve Analysis
Author(s) -
Udo Reischl,
Hans-Jörg Linde,
Norbert Lehn,
Olfert Landt,
K Barratt,
Nele Wellinghausen
Publication year - 2002
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.40.10.3814-3817.2002
Subject(s) - legionella pneumophila , legionella , microbiology and biotechnology , biology , legionnaires' disease , pneumonia , melting curve analysis , 16s ribosomal rna , polymerase chain reaction , bacteria , virology , gene , medicine , genetics
A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.