Open AccessPossible Approach for Serodiagnosis of Ascariasis by Evaluation of Immunoglobulin G4 Response UsingAscaris lumbricoidesSomatic Antigen
Author(s) -
Tanusree Bhattacharyya,
Amal Santra,
D. N. Majumder,
Bishnu P. Chatterjee
Publication year - 2001
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.39.8.2991-2994.2001
Subject(s) - ascaris lumbricoides , ascaris , ascariasis , antigen , immunoglobulin e , immunoglobulin m , ammonium sulfate , antibody , somatic antigen , biology , immunology , microbiology and biotechnology , immunoglobulin g , chemistry , chromatography , helminths
Somatic antigen of Ascaris lumbricoides was purified to homogeneity (molecular mass, 34 kDa) by ammonium sulfate fractionation and successive chromatographic procedures, namely, gel permeation, ion exchange, and high-performance gel permeation liquid chromatographies. The antigen showed strong binding with immunoglobulin G (IgG) in Ascaris-infested patients and was cross-reactive with IgE and IgG in patients infested with other nematodes. It reacted specifically with IgG4 (P < 0.001) in 63 Ascaris-infested patients, which represented 65% of the total IgG response, though cross-reactivity with IgG1, IgG2, and IgG3 subclasses was observed, indicating the unique specificity of this test system and its potential utility in the serodiagnosis of ascariasis.