Open AccessEndocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis
Author(s) -
Marissa B. Wilck,
WU Yan-yun,
John Greg Howe,
Jill Crouch,
Stephen C. Edberg
Publication year - 2001
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.39.5.2025-2027.2001
Subject(s) - endocarditis , biology , 16s ribosomal rna , polymerase chain reaction , dna sequencing , microbiology and biotechnology , dna , gene , staining , genetics , medicine , surgery
Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this method are discussed.