Open AccessEvaluation of a Group-Specific 16S Ribosomal DNA-Based PCR for Detection of Helicobacter bizzozeronii, Helicobacter felis , and Helicobacter salomonis in Fresh and Paraffin-Embedded Gastric Biopsy Specimens
Author(s) -
Dominic De Groote,
Freddy Haesebrouck,
LeenJan van Doorn,
Peter Vandamme,
Richard Ducatelle
Publication year - 2001
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.39.3.1197-1199.2001
Subject(s) - helicobacter , biology , 16s ribosomal rna , felis , ribosomal dna , polymerase chain reaction , ribosomal rna , microbiology and biotechnology , helicobacter pylori , dna , gastric biopsy , bacteria , gene , gastritis , medicine , genetics , cats , phylogenetics
A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens.