Open AccessImmunofluorescence Method for Detection of Ebola Virus Immunoglobulin G, Using HeLa Cells Which Express Recombinant Nucleoprotein
Author(s) -
Masayuki Saijo,
Masahiro Niikura,
Shigeru Morikawa,
Ichiro Kurane
Publication year - 2001
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.39.2.776-778.2001
Subject(s) - nucleoprotein , virology , ebola virus , immunofluorescence , antibody , recombinant dna , biology , virus , microbiology and biotechnology , hela , antigen , cell culture , immunology , gene , biochemistry , genetics
A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.