Open AccessComparison of Automated Ribotyping to Pulsed-Field Gel Electrophoresis for Genetic Fingerprinting of Streptococcus pneumoniae
Author(s) -
John Quale,
David Landman,
Carlos Flores,
Jayashree Ravishankar
Publication year - 2001
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.39.11.4175-4177.2001
Subject(s) - ribotyping , pulsed field gel electrophoresis , streptococcus pneumoniae , biology , gel electrophoresis , microbiology and biotechnology , dna profiling , typing , genotype , genetics , gene , dna , antibiotics
Fifty-two isolates of Streptococcus pneumoniae were characterized by pulsed-field gel electrophoresis (PFGE) and automated ribotyping by using HindIII and PvuII. HindIII ribotypes correlated well with PFGE. PvuII produced fewer bands and was less discriminatory. Automated ribotyping with HindIII is an accurate method for genetic fingerprinting of S. pneumoniae and can complement PFGE.