Rapid quantitation of DNA spot hybridization by flatbed scintillation counting | Zendy

Timo Hyypiä | Zendy; Eeva Auvinen | Zendy; S Kovanen | Zendy; T H Ståhlberg | Zendy

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Rapid quantitation of DNA spot hybridization by flatbed scintillation counting

Author(s) -

Timo Hyypiä,

Eeva Auvinen,

S Kovanen,

T H Ståhlberg

Publication year - 1990

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.28.1.159-162.1990

Subject(s) - scintillation , scintillation counter , dna–dna hybridization , hybridization probe , liquid scintillation counting , in situ hybridization , biology , dna , microbiology and biotechnology , physics , optics , chromatography , detector , chemistry , gene , genetics , gene expression

The flatbed scintillation counting system (Betaplate) was used for quantitative measurement of the radioactive hybridization signal in detection of adenovirus and papillomavirus DNA in clinical specimens. In this method, 96 samples on a nylon membrane can be handled as a single entity throughout the hybridization and counting procedure. The technique is sensitive, rapid, and convenient in routine use when compared with conventionally applied methods for the numerical analysis of hybridization results. The assay principle allows simultaneous testing of large numbers of specimens.

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