Sensitive chemiluminescent enzyme-linked immunosorbent assay for quantification of human immunoglobulin G and detection of herpes simplex virus | Zendy

A. D. Pronovost | Zendy; A. Baumgarten | Zendy; G. D. Hsiung | Zendy

Open Access

Sensitive chemiluminescent enzyme-linked immunosorbent assay for quantification of human immunoglobulin G and detection of herpes simplex virus

Author(s) -

A. D. Pronovost,

A. Baumgarten,

G. D. Hsiung

Publication year - 1981

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.13.1.97-101.1981

Subject(s) - herpes simplex virus , virology , chemiluminescence , antibody , virus , simplexvirus , biology , titer , immunoglobulin g , antigen , herpesviridae , virus isolation , viral disease , chemistry , immunology , chromatography

A chemiluminescent enzyme-linked immunosorbent assay (CELISA) was developed for detecting human immunoglobulin G and herpes simplex viral antigen. A comparison of CELISA with a conventional absorptiometric detection system showed that CELISA was 100 times more sensitive than absorptiometry for the measurement of human immunglobulin G. Similarly, CELISA detected as few as 40 plaque-forming units of herpes simplex virus in contrast to 2,500 plaque-forming units detected by absorptiometry. Of 18 specimens which were positive for herpes simplex virus type 1 by isolation in tissue culture, 15 (83%) were detected by CELISA within a few hours; in certain cases, several days were necessary for detection of virus by isolation techniques.

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