Open AccessComparative Study Using Phenotypic, Genotypic, and Proteomics Methods for Identification of Coagulase-Negative Staphylococci
Author(s) -
Anne J. M. Loonen,
A Jansz,
Jeandery N. B. Bergland,
Marion Valkenburg,
Petra Wolffs,
Adriaan J. C. van den Brule
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.06746-11
Subject(s) - coagulase , cons , genotype , biology , microbiology and biotechnology , identification (biology) , mass spectrometry , proteomics , staphylococcus , computational biology , genetics , gene , chromatography , chemistry , bacteria , staphylococcus aureus , computer science , botany , programming language
Five methods were compared to determine the most accurate method for identification of coagulase-negative staphylococci (CoNS) (n = 142 strains). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) showed the best results for rapid and accurate CoNS differentiation (99.3% of strains correctly identified). An alternative to this approach could be Vitek2 combined with partial tuf gene sequencing (100% of strains correctly identified when both methods are performed simultaneously).
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom