Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis of Gram-Positive, Catalase-Negative Cocci Not Belonging to the Streptococcus or Enterococcus Genus and Benefits of Database Extension | Zendy

Jens Jørgen Christensen | Zendy; Rimtas Dargis | Zendy; Monja Hammer | Zendy; Ulrik Stenz Justesen | Zendy; Xiaohui Chen Nielsen | Zendy; Michael Kemp | Zendy

Open Access

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis of Gram-Positive, Catalase-Negative Cocci Not Belonging to the Streptococcus or Enterococcus Genus and Benefits of Database Extension

Author(s) -

Jens Jørgen Christensen,

Rimtas Dargis,

Monja Hammer,

Ulrik Stenz Justesen,

Xiaohui Chen Nielsen,

Michael Kemp

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.06339-11

Subject(s) - gram positive cocci , mass spectrometry , database , enterococcus , gram , microbiology and biotechnology , matrix assisted laser desorption/ionization , streptococcus , enterococcus faecalis , biology , chemistry , bacteria , chromatography , desorption , computer science , staphylococcus aureus , antibiotics , organic chemistry , adsorption , genetics

Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.

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