Open AccessA Novel Real-Time PCR Method for Determination and Quantification of Each Cytomegalovirus Glycoprotein H Subtype in Clinical Samples
Author(s) -
Kazufumi Ikuta,
Ken Ishioka,
Yuka Sato,
Takashi Imamura,
Kimisato Asano,
Shin Koyano,
Naoki Inoue,
Tatsuo Suzutani
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.06032-11
Subject(s) - cytomegalovirus , virology , cytomegalovirus infections , human cytomegalovirus , epitope , real time polymerase chain reaction , betaherpesvirinae , biology , glycoprotein , herpesviridae , immunology , viral disease , antibody , virus , microbiology and biotechnology , gene , genetics
To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the CMV gH epitope region that can be used for evaluating pathogenic CMV strains in cases of mixed CMV infection.