Open AccessPulsed-Field Gel Electrophoresis Analysis of More than One Clinical Isolate of Campylobacter spp. from Each of 49 Patients in New Zealand
Author(s) -
Brent Gilpin,
Beth Robson,
Susan Lin,
Paula Scholes,
Stephen L. W. On
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.05928-11
Subject(s) - pulsed field gel electrophoresis , campylobacter , genotype , gel electrophoresis , biology , microbiology and biotechnology , dice , molecular epidemiology , genetics , bacteria , gene , statistics , mathematics
Pulsed-field gel electrophoresis (PFGE) analysis demonstrated that while 76% of patients had only one genotype of campylobacter, 10% carried two different but related genotypes (Dice coefficients > 0.78), and 14% carried at least two unrelated genotypes (Dice coefficients < 0.65). This supports the clustering of Campylobacter isolates with similar PFGE patterns, highlights the need to analyze multiple isolates from both sources and patients, and confirms that caution should be exercised before epidemiological links between patients or sources are dismissed.
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