Open AccessIdentification of Non-diphtheriae Corynebacterium by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Author(s) -
Adnan Alatoom,
Charles Cazanave,
Scott A. Cunningham,
Sherry M. Ihde,
Robin Patel
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.05889-11
Subject(s) - rpob , mass spectrometry , corynebacterium diphtheriae , corynebacterium , microbiology and biotechnology , matrix assisted laser desorption/ionization , chromatography , 16s ribosomal rna , identification (biology) , ribotyping , biology , chemistry , bacteria , polymerase chain reaction , desorption , diphtheria , genetics , virology , gene , vaccination , botany , organic chemistry , adsorption
We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of ≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom